PMID- 17940979
OWN - NLM
STAT- MEDLINE
DA  - 20071017
DCOM- 20080124
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 196 Suppl 2
DP  - 2007 Nov 15
TI  - Filovirus-like particles produced in insect cells: immunogenicity and protection 
      in rodents.
PG  - S421-9
AB  - BACKGROUND: Virus-like particles (VLPs) of Ebola virus (EBOV) and Marburg virus
      (MARV) produced in human 293T embryonic kidney cells have been shown to be
      effective vaccines against filoviral infection. In this study, we explored
      alternative strategies for production of filovirus-like particle-based vaccines, 
      to accelerate the development process. The goal of this work was to increase the 
      yield of VLPs, while retaining their immunogenic properties. METHODS: Ebola and
      Marburg VLPs (eVLPs and mVLPs, respectively) were generated by use of recombinant
      baculovirus constructs expressing glycoprotein, VP40 matrix protein, and
      nucleoprotein from coinfected insect cells. The baculovirus-derived eVLPs and
      mVLPs were characterized biochemically, and then the immune responses produced by
      the eVLPs in insect cells were studied further. RESULTS: The baculovirus-derived 
      eVLPs elicited maturation of human myeloid dendritic cells (DCs), indicating
      their immunogenic properties. Mice vaccinated with insect cell-derived eVLPs
      generated antibody and cellular responses equivalent to those vaccinated with
      mammalian 293T cell-derived eVLPs and were protected from EBOV challenge in a
      dose-dependent manner. CONCLUSION: Together, these data suggest that
      filovirus-like particles produced by baculovirus expression systems, which are
      amenable to large-scale production, are highly immunogenic and are suitable as
      safe and effective vaccines for the prevention of filoviral infection.
AD  - US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD
      21702, USA.
FAU - Warfield, Kelly L
AU  - Warfield KL
FAU - Posten, Nichole A
AU  - Posten NA
FAU - Swenson, Dana L
AU  - Swenson DL
FAU - Olinger, Gene G
AU  - Olinger GG
FAU - Esposito, Dominic
AU  - Esposito D
FAU - Gillette, William K
AU  - Gillette WK
FAU - Hopkins, Ralph F
AU  - Hopkins RF
FAU - Costantino, Julie
AU  - Costantino J
FAU - Panchal, Rekha G
AU  - Panchal RG
FAU - Hartley, James L
AU  - Hartley JL
FAU - Aman, M Javad
AU  - Aman MJ
FAU - Bavari, Sina
AU  - Bavari S
LA  - eng
GR  - N01-CO-12400/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
SB  - AIM
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Ebolavirus/immunology/physiology
MH  - Female
MH  - Filoviridae/immunology
MH  - Filoviridae Infections/*immunology
MH  - Hemorrhagic Fever, Ebola/*immunology
MH  - Humans
MH  - Marburg Virus Disease/*immunology
MH  - Marburgvirus/immunology/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rodentia
MH  - Virus Replication
EDAT- 2007/12/06 09:00
MHDA- 2008/01/25 09:00
CRDT- 2007/12/06 09:00
AID - JID38418 [pii]
AID - 10.1086/520612 [doi]
PST - ppublish
SO  - J Infect Dis. 2007 Nov 15;196 Suppl 2:S421-9.