PMID- 23252743
OWN - NLM
STAT- MEDLINE
DA  - 20130606
DCOM- 20130806
IS  - 1937-2345 (Electronic)
IS  - 0022-3395 (Linking)
VI  - 99
IP  - 3
DP  - 2013 Jun
TI  - Vaccination of mice with an antigenic serine protease-like protein elicits a
      protective immune response against Trichinella spiralis infection.
PG  - 426-32
LID - 10.1645/12-46.1 [doi]
AB  - Trichinellosis has major economic impacts on animal husbandry and food safety,
      and the control and elimination of trichinellosis is a major objective of
      veterinary medicine. A gene encoding serine protease of Trichinella spiralis
      (Ts-Adsp) was identified by immunoscreening an adult T. spiralis cDNA library. In
      this study, the recombinant Ts-Adsp protein (rTs-Adsp) was cloned and expressed
      in a prokaryotic expression system and purified by Ni-affinity chromatography. To
      determine whether the purified rTs-Adsp is a potential vaccine candidate for the 
      control of T. spiralis infection, we immunized BALB/c mice with this protein in
      combination with an alum adjuvant and subsequently challenged with T. spiralis
      larvae. The results showed that mice vaccinated with rTs-Adsp exhibited an
      average reduction in the muscle larvae burden of 46.5% relative to the control
      group. Immunization with the rTs-Adsp antigen induced both humoral and cellular
      immune responses, which manifested as elevated specific anti-rTs-Adsp IgG and IgE
      antibodies and a mixed Th1-Th2 response, as determined by Th1 (IFN-gamma and
      IL-2) and Th2 (IL-4, IL-10, and IL-13) cytokine profiling, with the Th2
      predominant. Thus, purified rTs-Adsp is able to limit the invasion of T. spiralis
      , and this protein could be an effective vaccine candidate for trichinellosis.
AD  - Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin
      University, Changchun, China.
FAU - Feng, Shuang
AU  - Feng S
FAU - Wu, Xiuping
AU  - Wu X
FAU - Wang, Xuelin
AU  - Wang X
FAU - Bai, Xue
AU  - Bai X
FAU - Shi, Haining
AU  - Shi H
FAU - Tang, Bin
AU  - Tang B
FAU - Liu, Xiaolei
AU  - Liu X
FAU - Song, Yanxia
AU  - Song Y
FAU - Boireau, Pascal
AU  - Boireau P
FAU - Wang, Feng
AU  - Wang F
FAU - Zhao, Ying
AU  - Zhao Y
FAU - Liu, Mingyuan
AU  - Liu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121219
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Helminth)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.- (Serine Proteases)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/blood
MH  - Antigens, Helminth/administration & dosage/genetics/*immunology
MH  - CD4-CD8 Ratio
MH  - Cytokines/metabolism
MH  - DNA, Complementary/administration & dosage/immunology
MH  - DNA, Helminth/administration & dosage/immunology
MH  - Female
MH  - Gene Expression Regulation
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/administration & dosage/immunology
MH  - Serine Proteases/administration & dosage/genetics/*immunology
MH  - Spleen/cytology/immunology
MH  - Trichinella spiralis/enzymology/genetics/*immunology
MH  - Trichinellosis/immunology/*prevention & control
MH  - Vaccination
MH  - *Vaccines, DNA/administration & dosage/immunology
EDAT- 2012/12/21 06:00
MHDA- 2013/08/07 06:00
CRDT- 2012/12/21 06:00
PHST- 2012/12/19 [aheadofprint]
AID - 10.1645/12-46.1 [doi]
PST - ppublish
SO  - J Parasitol. 2013 Jun;99(3):426-32. doi: 10.1645/12-46.1. Epub 2012 Dec 19.