PMID- 24142729
OWN - NLM
STAT- In-Data-Review
DA  - 20131021
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 33
IP  - 5
DP  - 2013 Oct
TI  - HPV16 E5 peptide vaccine in treatment of cervical cancer in vitro and in vivo.
PG  - 735-42
LID - 10.1007/s11596-013-1189-5 [doi]
AB  - Human papillomavirus (HPV)-induced cervical cancer is the second most common
      cancer among women worldwide. Despite the encouraging development of the
      preventive vaccine for HPV, a vaccine for both prevention and therapy or
      pre-cancerous lesions remains in high priority. Thus far, most of the HPV
      therapeutic vaccines are focused on HPV E6 and E7 oncogene. However these
      vaccines could not completely eradicate the lesions. Recently, HPV E5, which is
      considered as an oncogene, is getting more and more attention. In this study, we 
      predicted the epitopes of HPV16 E5 by bioinformatics as candidate peptide, then, 
      evaluated the efficacy and chose an effective one to do the further test. To
      evaluate the effect of vaccine, rTC-1 (TC-1 cells infected by rAAV-HPV16E5)
      served as cell tumor model and rTC-1 loading mice as an ectopic tumor model. We
      prepared vaccine by muscle injection. The vaccine effects were determined by
      evaluating the function of tumor-specific T cells by cell proliferation assay and
      ELISPOT, calculating the tumor volume in mice and estimating the survival time of
      mice. Our in vitro and in vivo studies revealed that injection of E5 peptide+CpG 
      resulted in strong cell-mediated immunity (CMI) and protected mice from tumor
      growth, meanwhile, prolonged the survival time after tumor cell loading. This
      study provides new insights into HPV16 E5 as a possible target on the therapeutic
      strategies about cervical cancer.
AD  - Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong
      University of Science and Technology, Wuhan, 430030, China,
      sjliao@tjh.tjmu.edu.cn.
FAU - Liao, Shu-Jie
AU  - Liao SJ
FAU - Deng, Dong-Rui
AU  - Deng DR
FAU - Zeng, Dan
AU  - Zeng D
FAU - Zhang, Ling
AU  - Zhang L
FAU - Hu, Xiao-Ji
AU  - Hu XJ
FAU - Zhang, Wei-Na
AU  - Zhang WN
FAU - Li, Li
AU  - Li L
FAU - Jiang, Xue-Feng
AU  - Jiang XF
FAU - Wang, Chang-Yu
AU  - Wang CY
FAU - Zhou, Jian-Feng
AU  - Zhou JF
FAU - Wang, Shi-Xuan
AU  - Wang SX
FAU - Zhang, Han-Wang
AU  - Zhang HW
FAU - Ma, Ding
AU  - Ma D
LA  - eng
PT  - Journal Article
DEP - 20131020
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
SB  - IM
EDAT- 2013/10/22 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/10/22 06:00
PHST- 2013/07/02 [received]
PHST- 2013/08/13 [revised]
PHST- 2013/10/20 [aheadofprint]
AID - 10.1007/s11596-013-1189-5 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2013 Oct;33(5):735-42. doi:
      10.1007/s11596-013-1189-5. Epub 2013 Oct 20.