PMID- 21956514
OWN - NLM
STAT- MEDLINE
DA  - 20110929
DCOM- 20120214
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 792
DP  - 2012
TI  - IL-7 addition increases spot size and number as measured by T-SPOT.TB ((R)).
PG  - 229-41
LID - 10.1007/978-1-61779-325-7_18 [doi]
AB  - The interferon-gamma (IFN-gamma) release assay (IGRA) is an in vitro extension of
      the century-old in vivo tuberculin skin test, better known as the TST.
      Shortcomings to the TST are multifactorial and include limitations in sensitivity
      and specificity. IGRAs improve diagnostic specificity by using antigens not found
      in the Bacille Calmette-Guerin, a vaccine given in most countries. IGRAs capture 
      the IFN-gamma produced by T cells in response to antigen stimulation. The ELISPOT
      immediately captures IFN-gamma produced directly from each cell, resulting in the
      generation of a cellular "footprint." The dimensions and intensity of the
      generated footprint indicate the avidity of the secreting cell. We show a further
      improvement in IGRAs by addition of interleukin-7 (IL-7). IL-7 reduces T-cell
      apoptosis and stabilizes IFN-gamma message. In addition to increasing the number 
      of spots in the ELISPOT T-SPOT.TB platform, IL-7 increased IFN-gamma production
      per cell as measured by an increase in spot size with no change in spot
      distribution.
AD  - Center for Molecular and Translational Human Infectious Disease
      Research/Molecular Tuberculosis Laboratory, Methodist Hospital Research
      Institute, Houston, TX, USA.
FAU - Feske, Marsha L
AU  - Feske ML
FAU - Medina, Miguel
AU  - Medina M
FAU - Graviss, Edward A
AU  - Graviss EA
FAU - Lewis, Dorothy E
AU  - Lewis DE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Interleukin-7)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Apoptosis
MH  - *Enzyme-Linked Immunospot Assay
MH  - Humans
MH  - Interferon-gamma/analysis/genetics/immunology/secretion
MH  - Interleukin-7/*immunology
MH  - RNA Stability
MH  - Recombinant Proteins/immunology
MH  - T-Lymphocytes/cytology/immunology/secretion
MH  - *Tuberculin Test
MH  - Tuberculosis/*diagnosis
EDAT- 2011/10/01 06:00
MHDA- 2012/02/15 06:00
CRDT- 2011/09/30 06:00
AID - 10.1007/978-1-61779-325-7_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;792:229-41. doi: 10.1007/978-1-61779-325-7_18.