PMID- 12167279
OWN - NLM
STAT- MEDLINE
DA  - 20020808
DCOM- 20020919
LR  - 20031114
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 18
IP  - 10
DP  - 2002 Jul 1
TI  - Epidermal powder immunization with a recombinant HIV gp120 targets Langerhans
      cells and induces enhanced immune responses.
PG  - 715-22
AB  - The recombinant envelope gp120 (rgp120) of human immunodeficiency virus (HIV) is 
      a weak immunogen when administered by intramuscular (IM) injection. In the
      present study, we report that epidermal powder immunization (EPI) elicits robust 
      antibody responses to the rgp120. EPI of mice with a dose 0.2-5 microg of rgp120 
      protein elicited geometric mean antibody titers that were 18- to 240-fold higher 
      than that elicited by IM injection using a 5.0 microg dose. Targeting antigen to 
      and mobilization of Langerhans cells (LCs) by EPI may explain the enhanced
      immunogenicity of the rgp120. EPI with rgp120 using sugar and gold particles as
      carrier resulted in differential antigen entry into the LCs and differential IgG 
      subclass antibody and cellular immune responses. EPI may serve as a useful tool
      to evaluate vaccine potential of the rgp120 protein.
AD  - PowderJect Vaccines, Inc., 585 Science Drive, Madison, WI 53711, USA.
      dexiang_chen@powderject.com
FAU - Chen, Dexiang
AU  - Chen D
FAU - Zuleger, C
AU  - Zuleger C
FAU - Chu, Q
AU  - Chu Q
FAU - Maa, Y-F
AU  - Maa YF
FAU - Osorio, J
AU  - Osorio J
FAU - Payne, L G
AU  - Payne LG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (AIDS Vaccines)
RN  - 0 (Carbohydrates)
RN  - 0 (HIV Antibodies)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Powders)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
SB  - X
MH  - AIDS Vaccines/*immunology
MH  - Administration, Cutaneous
MH  - Animals
MH  - Carbohydrates/*administration & dosage
MH  - Cell Movement
MH  - Female
MH  - HIV Antibodies/blood
MH  - HIV Envelope Protein gp120/*immunology
MH  - Immunization/*methods
MH  - Immunoglobulin G/blood/classification
MH  - Langerhans Cells/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Powders
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Vaccines, Synthetic/*immunology
EDAT- 2002/08/09 10:00
MHDA- 2002/09/20 10:01
CRDT- 2002/08/09 10:00
AID - 10.1089/088922202760072348 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2002 Jul 1;18(10):715-22.