PMID- 6260681
OWN - NLM
STAT- MEDLINE
DA  - 19810623
DCOM- 19810623
LR  - 20091118
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 32
IP  - 1
DP  - 1981 Apr
TI  - Mouse-protecting and histamine-sensitizing activities of pertussigen and fimbrial
      hemagglutinin from Bordetella pertussis.
PG  - 243-50
AB  - We compared the protective activities of fimbrial hemagglutinin (FHA) and
      pertussigen and their respective antibodies in mice infected intracerebrally with
      Bordetella pertussis. We found that mice were protected by a 1.7-microgram/mouse 
      dose of pertussigen which was free of detectable FHA and was detoxified by
      treatment with glutaraldehyde. A pertussigen preparation made from cells grown in
      shake cultures that did not contain demonstrable FHA protected mice at a dose of 
      1.4 microgram/mouse. FHA at a dose of 10 microgram/mouse protected mice from
      intracerebral infection, but it also sensitized mice to histamine at a dose of 2 
      micrograms/mouse, which indicated that it was contaminated with pertussigen. When
      FHA was obtained free of demonstrable pertussigen, it failed to sensitize mice to
      histamine at a dose of 30 micrograms/mouse and to protect mice from infection at 
      a dose of 12 micrograms/mouse (largest doses tested). Passive protection tests
      with antisera known to contain antibodies to pertussigen protected mice from
      intracerebral infection, whereas sera lacking anti-pertussigen antibodies but
      containing high concentrations of anti-FHA antibodies did not protect mice. The
      most important antigen for the immunization of mice against intracerebral
      infection with B. pertussis appears to be pertussigen.
FAU - Munoz, J J
AU  - Munoz JJ
FAU - Arai, H
AU  - Arai H
FAU - Cole, R L
AU  - Cole RL
LA  - eng
PT  - Journal Article
PL  - UNITED STATES
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Hemagglutinins)
RN  - 0 (Immune Sera)
RN  - 0 (Pertussis Vaccine)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 51-45-6 (Histamine)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Antigens, Bacterial
MH  - Bacterial Toxins/immunology/*therapeutic use
MH  - Bordetella pertussis/immunology
MH  - Hemagglutinins/*immunology
MH  - Histamine/*metabolism
MH  - Immune Sera/pharmacology
MH  - Immunity, Active
MH  - Mice
MH  - Pertussis Toxin
MH  - Pertussis Vaccine/immunology
MH  - Rabbits
MH  - Sheep
MH  - Virulence Factors, Bordetella
PMC - PMC350613
OID - NLM: PMC350613
EDAT- 1981/04/01
MHDA- 1981/04/01 00:01
CRDT- 1981/04/01 00:00
PST - ppublish
SO  - Infect Immun. 1981 Apr;32(1):243-50.