PMID- 6174666
OWN - NLM
STAT- MEDLINE
DA  - 19820521
DCOM- 19820521
LR  - 20091118
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 155
IP  - 4
DP  - 1982 Apr 1
TI  - Hybridoma antibodies against protective and nonprotective antigenic determinants 
      of a structurally defined polypeptide fragment of streptococcal M protein.
PG  - 1010-8
AB  - Hybridoma technology was used to produce a set of monoclonal antibodies against a
      purified polypeptide fragment of type 24 streptococcal M protein to delineate the
      protective determinants of M protein exposed on the surface of the virulent
      streptococci. Several hybridoma antibodies were found to be opsonic against the
      homologous type streptococci. At least two of these antibodies (IIC3.7 and
      IIC4.6) protected mice against challenge infections with the homologous, but not 
      a heterologous, serotype of bacteria. One of the hybridoma antibodies that
      reacted in high dilution (1:204,800) with the isolated M protein failed to react 
      with the M protein on the surface of type 24 streptococci, and thus did not
      opsonize the homologous organisms or protect mice against challenge infections.
      Because hybridoma antibodies are directed against a single distinct
      immunodeterminant, these results indicate that protective immunity may be
      directed at any one of several distinct antigenic determinants of M protein
      exposed on the surface of virulent group A streptococci.
FAU - Hasty, D L
AU  - Hasty DL
FAU - Beachey, E H
AU  - Beachey EH
FAU - Simpson, W A
AU  - Simpson WA
FAU - Dale, J B
AU  - Dale JB
LA  - eng
GR  - AI-10085/AI/NIAID NIH HHS/United States
GR  - DE-05062/DE/NIDCR NIH HHS/United States
GR  - IA-13550/PHS HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - UNITED STATES
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Peptides)
RN  - 0 (streptococcal M protein)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*biosynthesis
MH  - Antigen-Antibody Reactions
MH  - Antigens, Bacterial
MH  - *Bacterial Outer Membrane Proteins
MH  - Bacterial Proteins/*immunology
MH  - Blood Bactericidal Activity
MH  - *Carrier Proteins
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Epitopes
MH  - Female
MH  - Hybridomas/immunology
MH  - Immunization, Passive
MH  - Immunoglobulin G/classification
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Opsonin Proteins/immunology
MH  - Peptides/*immunology
MH  - Streptococcal Infections/immunology
MH  - Streptococcus pyogenes/immunology
PMC - PMC2186657
OID - NLM: PMC2186657
EDAT- 1982/04/01
MHDA- 1982/04/01 00:01
CRDT- 1982/04/01 00:00
PST - ppublish
SO  - J Exp Med. 1982 Apr 1;155(4):1010-8.