PMID- 2549344
OWN - NLM
STAT- MEDLINE
DA  - 19891005
DCOM- 19891005
LR  - 20031114
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 33
IP  - 4
DP  - 1989
TI  - Role of pertussigen (pertussis toxin) on the mouse protective activity of
      vaccines made from Bordetella species.
PG  - 341-55
AB  - Pertussigen [pertussis toxin (Ptx)] from Bordetella pertussis, when detoxified,
      induces protection in mice to intracerebral challenge (ic) with virulent B.
      pertussis. In its native form, minute nonprotective doses promote the development
      of immunity induced by other antigens of B. pertussis. As little as 4 ng of Ptx, 
      given with a nonprotective dose of 8 X 10(7) killed cells of the phase III
      Sakairi strain, promoted detectable protection to ic challenge. Native Ptx in
      doses of 0.4 to 400 ng did not protect mice, and vaccines made from strains not
      producing Ptx induced only weak protection. The marked enhancing action of Ptx
      was also observed with 5 micrograms of purified filamentous hemagglutinin and
      with vaccines made from other species of the Bordetella genus, such as B.
      parapertussis and B. bronchiseptica, but it was not observed with B. pertussis
      endotoxin. In addition, Ptx was still effective when given as late as 7 days
      after the vaccine. Antibodies to surface antigens of the challenge strain were
      demonstrated in sera of mice immunized with vaccines prepared with the different 
      Bordetella species tested, but antibodies to Ptx were detected only in the sera
      of mice immunized with the wild-type B. pertussis strains. Glutaraldehyde
      detoxified Ptx does not have this action. Pretreatment of normal mice with Ptx,
      also enhanced the protective action of a mouse antiserum to a wild-type strain of
      B. pertussis. These observations show that antigens other than Ptx are
      responsible for the protection, and that Ptx acts non-specifically to enhance the
      mouse protective action of those antigens.
AD  - Department of Health and Human Services, National Institute of Allergy and
      Infectious Diseases, Hamilton, Montana 59840.
FAU - Munoz, J J
AU  - Munoz JJ
FAU - Peacock, M G
AU  - Peacock MG
LA  - eng
PT  - Journal Article
PL  - JAPAN
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Surface)
RN  - 0 (Hemagglutinins)
RN  - 0 (Pertussis Vaccine)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Virulence Factors, Bordetella)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Adjuvants, Immunologic/immunology/*pharmacology
MH  - Animals
MH  - Antibodies, Bacterial/immunology
MH  - Antigens, Bacterial/immunology
MH  - Antigens, Surface/immunology
MH  - Bordetella Infections/immunology/prevention & control
MH  - Bordetella pertussis/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Hemagglutinins/pharmacology
MH  - Immunity/drug effects
MH  - Male
MH  - Mice
MH  - *Pertussis Toxin
MH  - Pertussis Vaccine/*immunology
MH  - Species Specificity
MH  - Vaccines, Inactivated/immunology
MH  - Virulence Factors, Bordetella/immunology/*pharmacology
EDAT- 1989/01/01
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PST - ppublish
SO  - Microbiol Immunol. 1989;33(4):341-55.