PMID- 2169577
OWN - NLM
STAT- MEDLINE
DA  - 19901019
DCOM- 19901019
LR  - 20070221
IS  - 0300-8584 (Print)
IS  - 0300-8584 (Linking)
VI  - 179
IP  - 3
DP  - 1990
TI  - Escherichia coli-derived envelope protein gD but not gC antigens of herpes
      simplex virus protect mice against a lethal challenge with HSV-1 and HSV-2.
PG  - 145-59
AB  - Immunization studies with HSV-1 and HSV-2 envelope proteins expressed in
      Escherichia coli were performed. After active immunization of mice with a gD-1
      antigen (Leu53-Ala312) expressed as a fusion protein, the animals were protected 
      from a lethal challenge with HSV-1 and HSV-2. In addition, antisera from rabbits 
      immunized with the same gD-1 antigen also conferred passive immunity to mice
      against a challenge infection with either HSV-1 or HSV-2. In contrast to these
      successful gD-1 protection experiments, various gC-1 and gC-2 fusion proteins
      from E. coli failed to induce protective immunity. Moreover, the mice sera from
      immunized animals were not able to react with the authentic, glycosylated gC-1
      and gC-2 envelope proteins, whereas sera raised against authentic gC-1 and gC-2
      glycoproteins do recognize the gC fusion proteins from E. coli. These results
      indicate, that E. coli might represent an ideal system for expressing gD antigens
      as a possible component of a HSV vaccine, whereas gC antigen cannot be produced
      in an immunocompetent form in E. coli.
AD  - Research Laboratories of Behringwerke AG, Marburg, Federal Republic of Germany.
FAU - Broker, M
AU  - Broker M
FAU - Abel, K J
AU  - Abel KJ
FAU - Kohler, R
AU  - Kohler R
FAU - Hilfenhaus, J
AU  - Hilfenhaus J
FAU - Amann, E
AU  - Amann E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - GERMANY, WEST
TA  - Med Microbiol Immunol
JT  - Medical microbiology and immunology
JID - 0314524
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein C, herpes simplex virus type 2)
RN  - 0 (glycoprotein D, Human herpesvirus 1)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/genetics/*immunology
MH  - DNA, Viral/analysis
MH  - Escherichia coli/genetics
MH  - Genes, Viral
MH  - Herpes Simplex/*prevention & control
MH  - Immunization, Passive
MH  - Mice
MH  - Recombinant Proteins/genetics/immunology
MH  - Simplexvirus/genetics/*immunology
MH  - Viral Envelope Proteins/genetics/*immunology
EDAT- 1990/01/01
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PST - ppublish
SO  - Med Microbiol Immunol. 1990;179(3):145-59.