PMID- 1433267 OWN - NLM STAT- MEDLINE DA - 19921201 DCOM- 19921201 LR - 20061115 IS - 0047-2565 (Print) IS - 0047-2565 (Linking) VI - 21 IP - 2-3 DP - 1992 Feb-May TI - Protection against SIV infection in macaques by immunization with inactivated virus from the BK28 molecular clone, but not with BK28-derived recombinant env and gag proteins. PG - 50-8 AB - Vaccination of cynomolgus macaques with beta-propiolactone inactivated SIVmacBK28 in Freund's adjuvant induced low but detectable levels of anti-SIV envelope (env) antibodies and T-cell responses and protected against challenge with the 32H isolate of SIVmac251 grown in C8166 cells. In contrast, purified recombinant SIV env and gag proteins derived from BK28 formulated in Syntex adjuvant generated consistent and long-lived cellular and humoral immune responses to SIV env, but failed to protect against infection with the 32H virus. Thus, protection against a heterogeneous challenge stock is possible by immunization with a molecularly-cloned virus, but not with recombinant proteins from the same molecular origin. High levels of anti-cell antibodies induced by the whole virus vaccine, but not by recombinant proteins, may have contributed to the protection observed. AD - National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, U.K. FAU - Mills, K H AU - Mills KH FAU - Page, M AU - Page M FAU - Chan, W L AU - Chan WL FAU - Kitchin, P AU - Kitchin P FAU - Stott, E J AU - Stott EJ FAU - Taffs, F AU - Taffs F FAU - Jones, W AU - Jones W FAU - Rose, J AU - Rose J FAU - Ling, C AU - Ling C FAU - Silvera, P AU - Silvera P AU - et al. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - DENMARK TA - J Med Primatol JT - Journal of medical primatology JID - 0320626 RN - 0 (Antibodies, Viral) RN - 0 (Gene Products, env) RN - 0 (Gene Products, gag) RN - 0 (Interleukin-2) RN - 0 (Vaccines, Inactivated) RN - 0 (Vaccines, Synthetic) RN - 0 (Viral Vaccines) SB - IM SB - X MH - Animals MH - Antibodies, Viral/biosynthesis MH - Enzyme-Linked Immunosorbent Assay MH - Gene Products, env/*immunology MH - Gene Products, gag/*immunology MH - Immunity, Cellular MH - Immunoblotting MH - Interleukin-2/biosynthesis MH - Lymphocyte Activation MH - *Macaca fascicularis MH - Neutralization Tests MH - Simian Acquired Immunodeficiency Syndrome/immunology/*prevention & control MH - Simian immunodeficiency virus/*immunology MH - T-Lymphocytes/immunology MH - Vaccination/veterinary MH - Vaccines, Inactivated/immunology MH - Vaccines, Synthetic/immunology MH - Viral Vaccines/immunology EDAT- 1992/02/01 MHDA- 1992/02/01 00:01 CRDT- 1992/02/01 00:00 PST - ppublish SO - J Med Primatol. 1992 Feb-May;21(2-3):50-8.