PMID- 1736531
OWN - NLM
STAT- MEDLINE
DA  - 19920312
DCOM- 19920312
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 187
IP  - 1
DP  - 1992 Mar
TI  - Recombinant vaccinia virus producing the prM and E proteins of yellow fever virus
      protects mice from lethal yellow fever encephalitis.
PG  - 290-7
AB  - Four recombinant vaccinia viruses were constructed for expression of different
      portions of the 17D yellow fever virus (YFV-17D) open reading frame. A
      recombinant, vP869, expressing prM and E induced high titers of neutralizing and 
      hemagglutination inhibiting antibodies in mice and was protective against
      intracranial challenge with the French neurotropic strain of YFV. Levels of
      protection were equivalent to those achieved by immunization with the YFV-17D
      vaccine virus. Recombinant vaccinia viruses expressing E and NS1, C prM, E, NS1, 
      or only NS1 failed to protect mice against challenge with YFV despite eliciting
      antibodies to NS1. The vP869-infected HeLa cells produced a particulate
      extracellular hemagglutinin (HA) similar to that produced by YFV-infected cells, 
      supporting previous studies with Japanese encephalitis virus (Mason et al.,
      1991), suggesting that the ability of recombinant vaccinia virus to produce
      extracellular HA particles is important for effective flavivirus immunity.
AD  - Virogenetics Corporation, Troy, New York 12180.
FAU - Pincus, S
AU  - Pincus S
FAU - Mason, P W
AU  - Mason PW
FAU - Konishi, E
AU  - Konishi E
FAU - Fonseca, B A
AU  - Fonseca BA
FAU - Shope, R E
AU  - Shope RE
FAU - Rice, C M
AU  - Rice CM
FAU - Paoletti, E
AU  - Paoletti E
LA  - eng
GR  - AI 10987-17/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - UNITED STATES
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Hemagglutinins, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/biosynthesis/immunology
MH  - Base Sequence
MH  - Cell Line
MH  - Hemagglutinins, Viral/genetics/*immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Precipitin Tests
MH  - Recombinant Proteins/genetics/immunology
MH  - Vaccines, Synthetic/immunology
MH  - Vaccinia virus/genetics/*immunology
MH  - Viral Proteins/genetics/*immunology
MH  - *Viral Vaccines/immunology
MH  - Yellow Fever/*prevention & control
MH  - Yellow fever virus/genetics/*immunology
EDAT- 1992/03/01
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PST - ppublish
SO  - Virology. 1992 Mar;187(1):290-7.