PMID- 1329705
OWN - NLM
STAT- MEDLINE
DA  - 19921125
DCOM- 19921125
LR  - 20071114
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 36
IP  - 3
DP  - 1992 Jul-Sep
TI  - Assessment of retrovirus-expressed nucleoprotein as a vaccine against lethal
      influenza virus infections of chickens.
PG  - 515-20
AB  - Hemagglutinin-based influenza vaccines stimulate protection in chickens that is
      limited to the serotype of the expressed hemagglutinin. To evaluate whether a
      more highly conserved influenza virus protein might stimulate a broader
      protective response, the influenza virus nucleoprotein (NP) was introduced into a
      retroviral vector (mRCAS/NP). NP is an internal influenza virus protein that has 
      been shown to stimulate cytotoxic T-cell responses in influenza-virus-infected
      mice. Cells infected with mRCAS/NP expressed approximately 10% of the level of NP
      observed in influenza-virus-infected chicken embryo fibroblasts. Immunocompetent 
      chicks were vaccinated intramuscularly with approximately 1 x 10(5) NP-expressing
      units of mRCAS/NP. Four weeks later, chicks were bled and challenged with a
      highly pathogenic avian influenza virus (A/Chicken/Victoria/1/85). The
      NP-expressing vector stimulated an influenza-virus-specific response, as
      indicated by the presence of antibody to NP, but failed to protect against the
      lethal challenge.
AD  - Department of Pathology, University of Massachusetts Medical Center, Worcester
      01655.
FAU - Brown, D W
AU  - Brown DW
FAU - Kawaoka, Y
AU  - Kawaoka Y
FAU - Webster, R G
AU  - Webster RG
FAU - Robinson, H L
AU  - Robinson HL
LA  - eng
GR  - AI 08831/AI/NIAID NIH HHS/United States
GR  - AI 29599/AI/NIAID NIH HHS/United States
GR  - CA 23086/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - UNITED STATES
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (Antibodies, Viral)
RN  - 0 (Influenza Vaccines)
RN  - 0 (NP protein, Influenza A virus)
RN  - 0 (Nucleoproteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Avian leukosis virus/metabolism
MH  - Chickens/*immunology
MH  - Genetic Vectors/immunology
MH  - Influenza A virus/*immunology
MH  - Influenza Vaccines/biosynthesis/*immunology
MH  - Influenza in Birds/immunology
MH  - Nucleoproteins/biosynthesis/*immunology
MH  - Poultry Diseases/*immunology/microbiology
MH  - *RNA-Binding Proteins
MH  - Vaccines, Synthetic/biosynthesis/immunology
MH  - Viral Core Proteins/biosynthesis/*immunology
EDAT- 1992/07/01
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PST - ppublish
SO  - Avian Dis. 1992 Jul-Sep;36(3):515-20.